Calvin Cotton, Ph.D.
Associate Professor
cuc@case.edu
BRB Room 825
(216) 368 4603 Phone
(216) 368 4223 Fax
Biography
Dr. Cotton graduated from Duke University with a BS degree in Zoology in 1977. He spent the following year at National Institute of Environmental Health Sciences and Mount Desert Island Biological Laboratory working with Dr. John Pritchard. He completed his graduate training at the University of North Carolina in the laboratories of Drs. John Gatzy and Richard Boucher and received his PhD in 1984. Dr. Cotton undertook postdoctoral training in the laboratory of Dr. Luis Reuss, Department of Physiology, Washington University and Department of Physiology and Biophysics, University of Texas Medical Branch. Dr. Cotton joined the faculty of the Departments of Pediatrics and Physiology and Biophysics at Case Western Reserve University in 1990.
Research Interests
The overall goal of our research is to develop an understanding the cell physiology of transepithelial salt and water transport and the regulation of these processes in health and disease. We are interested in both acute and chronic regulation of electrolyte transport by a variety of signal transduction pathways including those controlled by growth factors and hormones. Our studies currently involve two genetic disorders, cystic fibrosis (CF) and polycystic kidney disease (PKD), characterized by derangements in cell signaling and alterations epithelial ion transport. We utilize multiple types of biological preparations for our studies including: animals, tissue slices, primary cell cultures, immortalized cell lines, and transfected cells. Recently, we have taken advantage of a transgenic mouse that carries a temperature sensitive immortalizing gene to generate conditionally immortalized epithelial cell lines from relevant tissues of normal, PKD and CF mice. The resultant cell lines provide good model systems for exploring normal epithelial cell physiology and for examining the cellular mechanisms of pathophysiology associated with PKD and CF. Other transgenic mouse lines with tissue specific expression of green fluorescent protein or Cre are used to isolate pure populations of cells and to generate tissue specific knockouts of important molecules. Our laboratory is equipped to perform biochemical and molecular studies as well a functional evaluation of ion and water transport.
Publications
Falin RA, Cotton CU
"Acute downregulation of ENaC by EGF involves the PY motif and putative ERK phosphorylation site"
J Gen Physiol. 2007 Sep;130(3):313-28.
Veizis IE, Cotton CU
"Role of kidney chloride channels in health and disease."
Pediatr Nephrol. 2007 Jun;22(6):770-7. Epub 2006 Nov 16. Review.
Perez A, Issler AC, Cotton CU, Kelley TJ, Verkman AS, Davis PB
"CFTR inhibition mimics the cystic fibrosis inflammatory profile."
Am J Physiol Lung Cell Mol Physiol. 2007 Feb;292(2):L383-95. Epub 2006 Aug 18.
Falin R, Veizis IE, Cotton CU
"A role for ERK1/2 in EGF- and ATP-dependent regulation of amiloride-sensitive sodium absorption"
Am J Physiol Cell Physiol. 2005 May;288(5):C1003-11
Veizis IE, Cotton CU
"Abnormal EGF-dependent regulation of sodium absorption in ARPKD collecting duct cells"
Am J Physiol Renal Physiol. 2005 Mar;288(3):F474-82
Veizis EI, Carlin CR, Cotton CU
"Decreased amiloride-sensitive Na+ absorption in collecting duct principal cells isolated from BPK ARPKD mice"
Am J Physiol Renal Physiol. 2004 Feb;286(2):F244-54
Shen JP, Cotton CU
"Epidermal growth factor inhibits amiloride-sensitive sodium absorption in renal collecting duct cells"
Am J Physiol Renal Physiol. 2003 Jan;284(1):F57-64.
Cotton CU
"Development of conditionally immortalized epithelial cell lines from CF and non-CF mice"
Methods Mol Med. 2002;70:551-62
Sweeney WE Jr, Kusner L, Carlin CR, Chang S, Futey L, Cotton CU, Dell KM, Avner ED
"Phenotypic analysis of conditionally immortalized cells isolated from the BPK model of ARPKD"
Am J Physiol Cell Physiol. 2001 Nov;281(5):C1695-705.
Nakanishi K, Sweeney WE Jr, Macrae Dell K, Cotton CU, Avner ED
"Role of CFTR in autosomal recessive polycystic kidney disease"
J Am Soc Nephrol. 2001 Apr;12(4):719-25.
Gross E, Hawkins K, Abuladze N, Pushkin A, Cotton CU, Hopfer U, Kurtz I
"The stoichiometry of the electrogenic sodium bicarbonate cotransporter NBC1 is cell-type dependent"
J Physiol. 2001 Mar 15;531(Pt 3):597-603.
Takacs-Jarrett M, Sweeney WE, Avner ED, Cotton CU
"Generation & phenotype of cell lines derived from CF & non-CF mice that carry H-2K(b)-tsA58 transgene"
Am J Physiol Cell Physiol. 2001 Jan;280(1):C228-36.
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